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bioMerieux gmbh api20 strips
Api20 Strips, supplied by bioMerieux gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Mass Spectrometry:

Article Title: Antimicrobial resistance of Vibrio spp. from the coastal California system: discordance between genotypic and phenotypic patterns
Article Snippet: Frozen Vibrio spp. isolates banked on Microbank beads (Pro-Lab Diagnostics, Fisher Scientific, Round Rock, TX, USA) were plated on 5% sheep blood agar (Biological Media Services, University of California, Davis; Hardy Diagnostics, Santa Maria, CA, USA) at 37°C with 5% CO 2 for 24 hours. .. Isolates were clinically speciated using biochemical testing (API20E, BioMerieux, Durham, NC, USA), and a subset of isolates were confirmed by matrix-assisted laser desorption-ionization mass spectrometry (MALDI-ToF; Bruker Daltonics, Fremont, CA, USA) using the Bdal library in MBT Compass 4.1 ( ). .. DNA was extracted using the spin column protocol from the QiaAMP UCP Pathogen extraction kit with the additional mechanical pre-lysis protocol using small pathogen lysis tubes (Qiagen, Hilden, Germany).

Article Title: Antimicrobial resistance of Vibrio spp. from the coastal California system: discordance between genotypic and phenotypic patterns.
Article Snippet: Whole genome sequencing and genome assembly Frozen Vibrio spp. isolates banked on Microbank beads (Pro-Lab Diagnostics, Fisher Scientific, Round Rock, TX, USA) were plated on 5% sheep blood agar (Biological Media Services, University of California, Davis; Hardy Diagnostics, Santa Maria, CA, USA) at 37°C with 5% CO2 for 24 hours. .. Isolates were clinically speciated using biochemical testing (API20E, BioMerieux, Durham, NC, USA), and a subset of isolates were confirmed by matrix-assisted laser desorption-ionization mass spectrometry (MALDI-ToF; Bruker Daltonics, Fremont, CA, USA) using the Bdal library in MBT Compass 4.1 (33). .. DNA was extracted using the spin column protocol from the QiaAMP UCP Pathogen extraction kit with the additional mechanical pre-lysis protocol using small pathogen lysis tubes (Qiagen, Hilden, Germany).

Incubation:

Article Title: Survey on the Occurrence of Zoonotic Bacterial Pathogens in the Feces of Wolves (Canis lupus italicus) Collected in a Protected Area in Central Italy
Article Snippet: Feces were diluted 1:10 in Buffered Peptone Water (BPW) (Biolife) and, after mixing by stomacher, incubated at 37 ◦C for 24 h. Subsequently, 1 and 0.1 mL from BPW were subcultured in Selenite Broth (Biolife) and Rappaport Vassiliadis Broth (Biolife), respectively; Selenite Broth was incubated at 37 ◦C for 24 h, whereas Rappaport Vassiliadis Broth was incubated at 41.5 ◦C for 24 h. After incubation, 1 loopful from each broth was cultured on both Salmonella Shigella (SS) agar (Biolife) and Brilliant Green Agar (BGA) (Biolife) and incubated at 37 ◦C for 24 h. Suspected colonies were evaluated with conventional biochemical tests (TSI, urease, o-nitrofenil-β-D-galattopiranoside (ONPG), indole, VogesProskauer (VP), Lisyne decarboxylases and malonate) and confirmed as Salmonella spp. by PCR detection of invA gene [42]. .. Microorganisms 2024, 12, 2367 4 of 15 Shiga Toxin-Producing Escherichia coli (STEC) One loopful from BPW, after incubation, was cultured on Tryptone Bile X-glucuronide (TBX) agar (Biolife) and incubated at 42 ◦C for 24 h. Three distinct colonies were selected and subcultured on Tryptone Soy Agar (TSA) (Biolife) to be confirmed as E. coli by Api20E (Biomerieux). ..

Article Title: Survey on the Occurrence of Zoonotic Bacterial Pathogens in the Feces of Wolves ( Canis lupus italicus ) Collected in a Protected Area in Central Italy
Article Snippet: Feces were diluted 1:10 in Buffered Peptone Water (BPW) (Biolife) and, after mixing by stomacher, incubated at 37 °C for 24 h. Subsequently, 1 and 0.1 mL from BPW were subcultured in Selenite Broth (Biolife) and Rappaport Vassiliadis Broth (Biolife), respectively; Selenite Broth was incubated at 37 °C for 24 h, whereas Rappaport Vassiliadis Broth was incubated at 41.5 °C for 24 h. After incubation, 1 loopful from each broth was cultured on both Salmonella Shigella (SS) agar (Biolife) and Brilliant Green Agar (BGA) (Biolife) and incubated at 37 °C for 24 h. Suspected colonies were evaluated with conventional biochemical tests (TSI, urease, o-nitrofenil-β-D-galattopiranoside (ONPG), indole, Voges-Proskauer (VP), Lisyne decarboxylases and malonate) and confirmed as Salmonella spp. by PCR detection of invA gene [ ]. .. One loopful from BPW, after incubation, was cultured on Tryptone Bile X-glucuronide (TBX) agar (Biolife) and incubated at 42 °C for 24 h. Three distinct colonies were selected and subcultured on Tryptone Soy Agar (TSA) (Biolife) to be confirmed as E. coli by Api20E (Biomerieux). ..

Article Title: Phenotypic and genotypic characterization of antimicrobial resistance of non-typhoidal Salmonella from retail meat in California.
Article Snippet: Briefly, 50 g of each sample was placed into 250 ml buffered peptone water (BPW) (Becton Dickinson, Franklin Lakes, NJ, United States) in whirl-pak bags and homogenized by hand massaging or mechanical shaking for 3 min, followed by incubating the homogenates at 35 ◦C for 18–24 h. Then, 0.1 ml overnight enrichment was transferred to 10 ml RVR10 (Rappaport-Vassiliadis) and incubated at 42 ◦C for 20–24 h. The RVR10 enrichments were streaked onto XLT-4 (Remel, Lenexa, KS, United States). .. Two colonies of presumptive Salmonella based on colony morphology were then streaked onto blood agar plates (BAP) (Remel Inc., Lenexa, Kansas, United States) and incubated at 35 ◦C for 20–24 h. The API20E (bioMerieux Vitek, Inc., Hazelwood, Mo) was used in the Contra Costa Public Health Lab, while PCR was used in the UC Davis lab to confirm Salmonella. ..

Cell Culture:

Article Title: Survey on the Occurrence of Zoonotic Bacterial Pathogens in the Feces of Wolves (Canis lupus italicus) Collected in a Protected Area in Central Italy
Article Snippet: Feces were diluted 1:10 in Buffered Peptone Water (BPW) (Biolife) and, after mixing by stomacher, incubated at 37 ◦C for 24 h. Subsequently, 1 and 0.1 mL from BPW were subcultured in Selenite Broth (Biolife) and Rappaport Vassiliadis Broth (Biolife), respectively; Selenite Broth was incubated at 37 ◦C for 24 h, whereas Rappaport Vassiliadis Broth was incubated at 41.5 ◦C for 24 h. After incubation, 1 loopful from each broth was cultured on both Salmonella Shigella (SS) agar (Biolife) and Brilliant Green Agar (BGA) (Biolife) and incubated at 37 ◦C for 24 h. Suspected colonies were evaluated with conventional biochemical tests (TSI, urease, o-nitrofenil-β-D-galattopiranoside (ONPG), indole, VogesProskauer (VP), Lisyne decarboxylases and malonate) and confirmed as Salmonella spp. by PCR detection of invA gene [42]. .. Microorganisms 2024, 12, 2367 4 of 15 Shiga Toxin-Producing Escherichia coli (STEC) One loopful from BPW, after incubation, was cultured on Tryptone Bile X-glucuronide (TBX) agar (Biolife) and incubated at 42 ◦C for 24 h. Three distinct colonies were selected and subcultured on Tryptone Soy Agar (TSA) (Biolife) to be confirmed as E. coli by Api20E (Biomerieux). ..

Article Title: Survey on the Occurrence of Zoonotic Bacterial Pathogens in the Feces of Wolves ( Canis lupus italicus ) Collected in a Protected Area in Central Italy
Article Snippet: Feces were diluted 1:10 in Buffered Peptone Water (BPW) (Biolife) and, after mixing by stomacher, incubated at 37 °C for 24 h. Subsequently, 1 and 0.1 mL from BPW were subcultured in Selenite Broth (Biolife) and Rappaport Vassiliadis Broth (Biolife), respectively; Selenite Broth was incubated at 37 °C for 24 h, whereas Rappaport Vassiliadis Broth was incubated at 41.5 °C for 24 h. After incubation, 1 loopful from each broth was cultured on both Salmonella Shigella (SS) agar (Biolife) and Brilliant Green Agar (BGA) (Biolife) and incubated at 37 °C for 24 h. Suspected colonies were evaluated with conventional biochemical tests (TSI, urease, o-nitrofenil-β-D-galattopiranoside (ONPG), indole, Voges-Proskauer (VP), Lisyne decarboxylases and malonate) and confirmed as Salmonella spp. by PCR detection of invA gene [ ]. .. One loopful from BPW, after incubation, was cultured on Tryptone Bile X-glucuronide (TBX) agar (Biolife) and incubated at 42 °C for 24 h. Three distinct colonies were selected and subcultured on Tryptone Soy Agar (TSA) (Biolife) to be confirmed as E. coli by Api20E (Biomerieux). ..

other:

Article Title: A retrospective investigation of the population structure and geospatial distribution of Salmonella Paratyphi A in Kathmandu, Nepal
Article Snippet: Salmonella colonies were identified using API20E (bioMerieux, Inc) and confirmed using the same serotype-specific antisera as above.

Article Title: Building of a new Spectra for the identification of Phytobacter spp., an emerging Enterobacterales, using MALDI Biotyper
Article Snippet: Reviewer 2 – Review Attachment 2 _____________________________ “manual biochemical tests, different automation panels, and MALDI-TOF” 1) give examples (e.g. API20/50, Phoenix100, Bruker/Saramis) Examples added to the text: r/b tubes, API20E, Vitek GN panels, Microscan Plates, and bioMerieux MALDI-TOF-MS ________________________________________________________________________________ “naming these isolates equivocally as” 2) assigning these isolates mistakingly to Text modified accordingly ________________________________________________________________________ “Even sequencing methods”... 2) when not properly implemented Text modified accordingly.

Polymerase Chain Reaction:

Article Title: Phenotypic and genotypic characterization of antimicrobial resistance of non-typhoidal Salmonella from retail meat in California.
Article Snippet: Briefly, 50 g of each sample was placed into 250 ml buffered peptone water (BPW) (Becton Dickinson, Franklin Lakes, NJ, United States) in whirl-pak bags and homogenized by hand massaging or mechanical shaking for 3 min, followed by incubating the homogenates at 35 ◦C for 18–24 h. Then, 0.1 ml overnight enrichment was transferred to 10 ml RVR10 (Rappaport-Vassiliadis) and incubated at 42 ◦C for 20–24 h. The RVR10 enrichments were streaked onto XLT-4 (Remel, Lenexa, KS, United States). .. Two colonies of presumptive Salmonella based on colony morphology were then streaked onto blood agar plates (BAP) (Remel Inc., Lenexa, Kansas, United States) and incubated at 35 ◦C for 20–24 h. The API20E (bioMerieux Vitek, Inc., Hazelwood, Mo) was used in the Contra Costa Public Health Lab, while PCR was used in the UC Davis lab to confirm Salmonella. ..



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